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Immunology and Infection

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JoVE Journal Immunology and Infection
Oprensning og visualisering af lipopolysaccharid fra Gram-negative bakterier ved varm vandig-phenolekstraktion
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Oprensning og visualisering af lipopolysaccharid fra Gram-negative bakterier ved varm vandig-phenolekstraktion

Article DOI: 10.3791/3916-v • 05:32 min • May 28th, 2012
May 28th, 2012 •
1, 1
1Department of Microbiology, Immunology, & Cancer Biology, University of Virginia Health System

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Vi beskriver en modificeret varm vandig ekstraktion med phenol fremgangsmåde til oprensning af lipopolysaccharid (LPS) fra gramnegative bakterier. Når først ekstraheres, kan de LPS efterfølgende analyseret ved SDS-PAGE og visualiseret ved direkte farvning eller Western immunoblot.

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Immunologi Gram-negativ LPS ekstraktion polysaccharid farvning Western-immunblot
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