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热酚水提取纯化及革兰氏阴性菌脂多糖的可视化
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction
DOI:

05:31 min

May 28, 2012

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Chapters

  • 00:05Title
  • 01:38Preparation of Bacteria and LPS Extraction
  • 04:21LPS Purification Results
  • 04:48Conclusion

Summary

Automatic Translation

我们描述一个净化革兰氏阴性菌脂多糖(LPS)的修改热水溶液中苯酚的提取方法。一旦提取,内毒素可随后通过SDS-PAGE分析,并通过可视化的直接染色或西方免疫。

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