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Immunology and Infection
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热酚水提取纯化及革兰氏阴性菌脂多糖的可视化
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction
Please note that all translations are automatically generated.
Click here for the English version.
热酚水提取纯化及革兰氏阴性菌脂多糖的可视化
DOI:
10.3791/3916-v
•
05:31 min
•
May 28, 2012
•
Michael R. Davis, Jr.
,
Joanna B. Goldberg
1
Department of Microbiology, Immunology, & Cancer Biology
,
University of Virginia Health System
Chapters
00:05
Title
01:38
Preparation of Bacteria and LPS Extraction
04:21
LPS Purification Results
04:48
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述一个净化革兰氏阴性菌脂多糖（LPS）的修改热水溶液中苯酚的提取方法。一旦提取，内毒素可随后通过SDS-PAGE分析，并通过可视化的直接染色或西方免疫。
Tags
Purification
Visualization
Lipopolysaccharide
Gram-negative Bacteria
Hot Aqueous-phenol Extraction
Lipid A
Core Oligosaccharide
O-antigen Units
Virulence
Pathogenesis
Serotyping
Clinical Isolates
Antibodies
Mutants
Extraction Method
Nucleic Acids
Proteins
Sodium Dodecy
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