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Immunology and Infection

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JoVE Journal Immunology and Infection
Affinitetsrening av ribonukleoprotein influensavirus komplex från kromatinet hos infekterade celler
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Affinitetsrening av ribonukleoprotein influensavirus komplex från kromatinet hos infekterade celler

Article DOI: 10.3791/4028-v • 11:20 min • June 3rd, 2012
June 3rd, 2012 •
1, 1
1Department of Virology, Universitätsklinikum Freiburg

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Influensavirus replikera sitt RNA-genom i association med värd-cell-kromatin. Här presenterar vi en metod för att rena intakta virala ribonukleoproteinpartiklar komplex från kromatin av infekterade celler. Renat virus komplex kan analyseras genom både Western blöt och primerförlängning av protein och RNA-halt, respektive.

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Virologi Molecular Biology influensa A-virus affinitetsrening en subcellulär fraktionering kromatin vRNP komplex polymeras
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