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Affinitetsrensning af Influenzavirus ribonukleoprotein komplekser fra kromatin af inficerede celler
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Chapters
- 00:05Title
- 02:12Subcellular Fractionation
- 06:38Strep-tag Purification
- 08:39Analysis of Purified Ribonucleoprotein Complexes
- 10:38Conclusion
Influenzavirus replikere deres RNA-genom i association med værts-celle kromatin. Her præsenterer vi en metode til at rense intakte virale ribonucleoprotein komplekser fra kromatin af inficerede celler. Oprenset virus komplekser kan analyseres ved både Western blot og primerforlængelse af protein og RNA-indhold, hhv.
Tags
Affinity PurificationInfluenza VirusRibonucleoprotein ComplexesChromatinNegative-strand RNA VirusesViral GenomeNucleoproteinPolymerase ComplexViral RNA SynthesisViral MRNA SynthesisCellular Pre-mRNAsChromatin InteractionsHost RNA Polymerase IIHost NucleosomesRecombinant Influenza VirusesOne-Strep-TagPB2 SubunitAffinity Purification Of VRNPsLysis Procedures
