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Neuroscience

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Preparation of Drosophila Central Neurons for in situ Patch Clamping
 

Preparation of Drosophila Central Neurons for in situ Patch Clamping

Article doi: 10.3791/4264
October 15th, 2012

Summary October 15th, 2012

In situ patch clamp recordings are used for electrophysiological characterization of neurons in intact circuitry. In the Drosophila genetic model patch clamping is difficult because the CNS is small and surrounded by a robust sheath. This article describes the procedure to remove the sheath and clean neurons for subsequent patch clamp recordings.

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