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JoVE Journal Neuroscience
Preparação de
Induction of Complete Transection-Type Spinal Cord Injury in Mice
Journal (Neuroscience)
Induction of Complete Transection-Type Spinal Cord Injury in Mice
Paired Nanoinjection and Electrophysiology Assay to Screen for Bioactivity of Compounds using the Drosophila melanogaster Giant Fiber System
Journal (Neuroscience)
Paired Nanoinjection and Electrophysiology Assay to Screen for Bioactivity of Compounds using the Drosophila melanogaster Giant Fiber System
 
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Preparação de

Article DOI: 10.3791/4264 • 08:27 min
October 15th, 2012 •
1, 1
1School of Life Sciences, Arizona State University

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October 15th, 2012

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No patch situ gravações de fixação são usados ​​para a caracterização eletrofisiológica dos neurônios no circuito intacto. No modelo genético Drosophila patch clamping é difícil, porque o SNC é pequeno e está rodeado por uma bainha robusto. Este artigo descreve o procedimento para remover os neurônios bainha e limpa para gravações posteriores braçadeira de patches.

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