Preparation of Acute Spinal Cord Slices for Whole-cell Patch-clamp Recording in Substantia Gelatinosa Neurons

Mengye Zhu; Daying Zhang; Sicong Peng; Nana Liu; Jing Wu; Haixia Kuang; Tao Liu

doi:10.3791/58479

Journal

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Neuroscience

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Preparation of Acute Spinal Cord Slices for Whole-cell Patch-clamp Recording in Substantia Gelatinosa Neurons

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Preparation of Acute Spinal Cord Slices for Whole-cell Patch-clamp Recording in Substantia Gelatinosa Neurons

14,045 Views

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08:30 min

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January 18, 2019

DOI:

10.3791/58479-v

DOI:

10.3791/58479-v

•

08:30 min

January 18, 2019

•

14000 Views

Mengye Zhu¹, Daying Zhang¹, Sicong Peng², Nana Liu², Jing Wu², Haixia Kuang², Tao Liu^2,3

¹Department of Pain,First Affiliated Hospital of Nanchang University ²Department of Pediatrics,First Affiliated Hospital of Nanchang University ³Center for Laboratory Medicine,First Affiliated Hospital of Nanchang University

Summary

Here, we describe the essential steps for whole-cell patch-clamp recordings made from substantia gelatinosa (SG) neurons in the in vitro spinal cord slice. This method allows the intrinsic membrane properties, synaptic transmission and morphological characterization of SG neurons to be studied.