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制备<em>果蝇</em>中央神经元<em在原位</em>膜片钳
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Neuroscience
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JoVE Journal Neuroscience
Preparation of Drosophila Central Neurons for in situ Patch Clamping
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制备<em>果蝇</em>中央神经元<em在原位</em>膜片钳

DOI:
10.3791/4264-v

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08:27 min

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October 15, 2012

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,
1School of Life Sciences,Arizona State University

Chapters

  • 00:05Title
  • 02:10Visualization of MN5
  • 03:21Enzyme Pipette Preparation
  • 05:08Cleaning of GFP-tagged MN5
  • 07:02Results: In situ Patch Clamping of Drosophila Central Neurons
  • 07:57Conclusion

Summary

Automatic Translation

Automatic Translation

原位补丁钳用于神经元的电生理特性的完整电路。在果蝇的遗传模型膜片钳是困难的，因为中枢神经系统，周围环绕着一个强大的护套。本文介绍的步骤，取出护套和清洁随后的膜片钳记录神经元。

Tags

Drosophila Central NeuronsIn Situ Patch ClampingFruit Fly Drosophila MelanogasterGenetic ModelIon ChannelsNeuronal ExcitabilityVoltage Gated Potassium Channel ShakerGenetic ToolsPatch Clamp RecordingsDrosophila CNSGlia And Collagen SheathPatch Pipette AccessAdult Drosophila CNSIn Situ Patch Clamp RecordingsAdult BrainVentral Nerve CordStable Giga-sealWhole Cell Patch Clamp Recordings

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