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Fremstilling af<em> Drosophila</em> Centrale neuroner for<em> In situ</em> Patch Clamping
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Chapters
- 00:05Title
- 02:10Visualization of MN5
- 03:21Enzyme Pipette Preparation
- 05:08Cleaning of GFP-tagged MN5
- 07:02Results: In situ Patch Clamping of Drosophila Central Neurons
- 07:57Conclusion
In situ patch clamp-optagelser anvendes til elektrofysiologisk karakterisering af neuroner i intakt kredsløb. I Drosophila genetisk model patch fastspænding er vanskelig, fordi de CNS er lille og omgivet af en robust kappe. Denne artikel beskriver procedure for at fjerne hylsteret og rene neuroner for efterfølgende patch clamp optagelser.
Tags
Drosophila Central NeuronsIn Situ Patch ClampingFruit Fly Drosophila MelanogasterGenetic ModelIon ChannelsNeuronal ExcitabilityVoltage Gated Potassium Channel ShakerGenetic ToolsPatch Clamp RecordingsDrosophila CNSGlia And Collagen SheathPatch Pipette AccessAdult Drosophila CNSIn Situ Patch Clamp RecordingsAdult BrainVentral Nerve CordStable Giga-sealWhole Cell Patch Clamp Recordings
