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JoVE Journal Neuroscience
Sequencial de foto-branqueamento de delinear células de Schwann individuais na junção neuromuscular
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Sequencial de foto-branqueamento de delinear células de Schwann individuais na junção neuromuscular

Article DOI: 10.3791/4460-v • 11:22 min • January 11th, 2013
January 11th, 2013 •
1, 1, 1,2,3,4
1Lehrstuhl für Biomolekulare Sensoren, Technische Universität München, 2Center for Integrated Protein Science (Munich) at the Institute of Neuroscience, Technische Universität München, 3TUM Institute for Advanced Study and German Center for Neurodegenerative Diseases, Technische Universität München, 4Munich Cluster for Systems Neurology (SyNergy), Technische Universität München

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Visualizando as células individuais em tecidos densamente compactados, tais como as células de Schwann terminais (SCs) em junções neuromusculares (NMJs), é um desafio. "Sequential foto-branqueamento" permite delinear SCs terminais individuais, por exemplo, no explante triangularis músculo Sterni, uma preparação conveniente nervo-músculo, onde sequencial de branqueamento podem ser combinados com o lapso de tempo de imagem e

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Neurociência Edição 71 Biologia Celular Biologia Molecular Neurobiologia Imunologia Medicina Anatomia Fisiologia Cirurgia Triangularis Sterni explante as células de Schwann de imagem junção neuromuscular imunohistoquímica branqueamento músculos nervos mouse modelo animal,
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