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JoVE Journal Neuroscience
I ovo Expression av mikroRNA i ventral Chick midthjernen
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I ovo Expression av mikroRNA i ventral Chick midthjernen

Article DOI: 10.3791/50024-v • 09:19 min • September 16th, 2013
September 16th, 2013 •
1, 1, 1, 1, 1, 1, 1
1Department of Experimental Embryology, Institute of Anatomy, University of Tübingen

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Ektopisk uttrykk er en teknikk for å belyse den microRNAs rolle i hjernens utvikling. Men, rettet mot bestemte områder ved hjelp av i ovo electroporation er utfordrende. Her viser vi en effektiv måte å selektivt electroporate ventrale og dorsale midthjernen regioner.

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Neuroscience Central Nervous System nevral utvikling kylling embryo mikroRNA elektroporering
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