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Neuroscience

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JoVE Journal Neuroscience
Dissekere celle-autonom funksjon av skjøre X mental retardasjon protein i en auditiv krets ved i ovo elektroporering
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Dissekere celle-autonom funksjon av skjøre X mental retardasjon protein i en auditiv krets ved i ovo elektroporering

Article DOI: 10.3791/64187-v • 11:10 min • July 6th, 2022
July 6th, 2022 •
*1, *2, 3, 1
1Division of Histology & Embryology, Key Laboratory for Regenerative Medicine of the Ministry of Education, College of Medicine, Jinan University, 2Department of Clinical Pathology, First Affiliated Hospital of Jinan University, 3Program in Neuroscience, Department of Biomedical Sciences, College of Medicine, Florida State University
* These authors contributed equally

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Ved hjelp av ovo-elektroporering utviklet vi en metode for selektivt å transfektere det hørbare indre øret og cochleakjernen i kyllingembryoer for å oppnå en cellegruppespesifikk knockdown av skjørt X-mentalt retardasjonsprotein i diskrete perioder med kretsmontering.

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Nevrovitenskap utgave 185 fragilt X-syndrom sensorisk krets indre øre cochleakjerne synapsedannelse hjerneutvikling endbulb av Held kyllingembryo
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