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JoVE Journal Neuroscience
Analysere DNA-skader i hippocampus Neurons kometsystemet
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Analysere DNA-skader i hippocampus Neurons kometsystemet

Article DOI: 10.3791/50049 • 10:00 min • December 19th, 2012
December 19th, 2012 •
1, 2, 1,3
1Department of Radiation Oncology, University of Alabama-Birmingham, 2Department of Radiation Oncology, The Ohio State University Medical School, 3Department of Cell Biology, and Pharmacology and Toxicology, Comprehensive Cancer Center, University of Alabama at Birmingham School of Medicine, University of Alabama-Birmingham

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Kometen analysen er en effektiv måte å oppdage enkelt-og dobbelt-tråd pauser, inkludert alkali-labile områder og DNA-DNA/DNA-protein kryssbindinger på DNA i alle celler, inkludert hippocampus nevroner. Metoden utnytter differensial migrasjon av DNA i et elektrisk felt på grunn av forskjeller i mengden av DNA-skade.

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Nevrovitenskap genetikk cellebiologi medisin kreft biologi anatomi fysiologi DNA DNA-skade dobbel tråd pause enkelt strand pause reparasjon nevroner kometmetode cellekultur
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