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23 articles published in JoVE
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Mechanical Ventilation Boot Camp Curriculum
Jennifer Yee1,2, Alma Benner2, Jared Hammond2, Bethany Malone2, Charles Fuenning3, Richard George2,4, Rami A. Ahmed2,3,4
1Department of Emergency Medicine, The Ohio State University, 2Summa Health System, Akron City Hospital, 3Western Reserve Hospital, 4Northeast Ohio Medical University
This curriculum outlines how to execute a simulation-based boot camp to teach providers how to manage mechanically ventilated patients.
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The Calibration and Use of Capacitance Sensors to Monitor Stem Water Content in Trees
Ashley M. Matheny1, Steven R. Garrity2, Gil Bohrer3
1Department of Geological Sciences, Jackson School of Geosciences, University of Texas at Austin, 2METER Group, Inc., USA, 3Department of Civil, Environmental and Geodetic Engineering, Ohio State University
The hydraulic capacitance of biomass is a key component of the vegetation water budget, which serves as a buffer against short and long-term drought stresses. Here, we present a protocol for the calibration and use of soil moisture capacitance sensors to monitor water content in the stems of large trees.
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Standardized Method for High-throughput Sterilization of Arabidopsis Seeds
Benson E. Lindsey III*1, Luz Rivero*1, Chistopher S. Calhoun1, Erich Grotewold1,2, Jelena Brkljacic1
1Arabidopsis Biological Resource Center, Center for Applied Plant Sciences, The Ohio State University, 2Department of Molecular Genetics, Center for Applied Plant Sciences, The Ohio State University
The objective of this study was to determine the effects of bleach and chlorine gas sterilization on seed germination of a range of Arabidopsis genotypes grown on sterile media. Optimized sterilization protocols have been developed to prevent the growth of microbial contaminants while providing satisfactory seed survival.
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Bidirectional Retroviral Integration Site PCR Methodology and Quantitative Data Analysis Workflow
Gajendra W. Suryawanshi*1,2, Song Xu*3, Yiming Xie1, Tom Chou3, Namshin Kim4, Irvin S. Y. Chen1,5, Sanggu Kim6
1UCLA AIDS Institute, University of California at Los Angeles (UCLA), 2Department of Microbiology, Immunology, & Molecular Genetics, University of California at Los Angeles (UCLA), 3Departments of Biomathematics and Mathematics, University of California at Los Angeles (UCLA), 4Personalized Genomic Medicine Research Center, Division of Strategic Research Groups, Korea Research Institute of Bioscience and Biotechnology, 5Department of Medicine, University of California at Los Angeles (UCLA), 6Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University (OSU)
This manuscript describes the experimental procedure and software analysis for a bidirectional integration site assay that can simultaneously analyze upstream and downstream vector-host junction DNA. Bidirectional PCR products can be used for any downstream sequencing platform. The resulting data are useful for a high-throughput, quantitative comparison of integrated DNA targets.
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Assaying DNA Damage in Hippocampal Neurons Using the Comet Assay
Somaira Nowsheen1, Fen Xia2, Eddy S. Yang1,3
1Department of Radiation Oncology, University of Alabama-Birmingham, 2Department of Radiation Oncology, The Ohio State University Medical School, 3Department of Cell Biology, and Pharmacology and Toxicology, Comprehensive Cancer Center, University of Alabama at Birmingham School of Medicine, University of Alabama-Birmingham
The comet assay is an efficient way of detecting single- and double-strand breaks, including alkali-labile sites and DNA-DNA/DNA-protein cross-links on the DNA in all cells including hippocampal neurons. The method takes advantage of the differential migration of DNA in an electric field due to differences in amount of DNA damage.
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Collection, Isolation and Enrichment of Naturally Occurring Magnetotactic Bacteria from the Environment
Zachery Oestreicher1, Steven K. Lower1,2, Wei Lin3, Brian H. Lower2
1School of Earth Sciences, The Ohio State University, 2School of Environment & Natural Resources, The Ohio State University, 3Institute of Geology and Geophysics, Chinese Academy of Sciences
We demonstrate a method to collect magnetotactic bacteria (MTB) that can be applied to natural waters. MTB can be isolated and enriched from sediment samples using a relatively simple setup that takes advantage of the bacteria's natural magnetism. Isolated MTB can then be examined in detail using both light and electron microscopy.
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Ex Vivo Assessment of Contractility, Fatigability and Alternans in Isolated Skeletal Muscles
Ki Ho Park1, Leticia Brotto2, Oanh Lehoang1, Marco Brotto2, Jianjie Ma1, Xiaoli Zhao1,3
1Department of Physiology and Biophysics, UMDNJ-Robert Wood Johnson Medical School, 2Muscle Biology Research Group, University of Missouri-Kansas City, 3Pharmacology division, College of Pharmacy, DHLRI, Ohio State University
We describe a method to directly measure muscle force, muscle power, contractile kinetics and fatigability of isolated skeletal muscles in an in vitro system using field stimulation. Valuable information on Ca2+ handling properties and contractile machinery of the muscle can be obtained using different stimulating protocols.
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Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps
Bhavani Gopalakrishnan1, Kevin M. Nash1, Murugesan Velayutham1, Frederick A. Villamena1,2
1The Davis Heart and Lung Research Institute, The Ohio State University, 2Department of Pharmacology, College of Medicine, The Ohio State University
Electron paramagnetic resonance (EPR) spectroscopy was employed to detect nitric oxide from bovine aortic endothelial cells and superoxide radical anion from human neutrophils using iron (II)-N-methyl-D-glucamine dithiocarbamate, Fe(MGD)2 and 5,5-dimethyl-1-pyroroline-N-oxide, DMPO, respectively.
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Enabling High Grayscale Resolution Displays and Accurate Response Time Measurements on Conventional Computers
Xiangrui Li1, Zhong-Lin Lu2,3,4,5
1Center for Cognitive and Behavioral Brain Imaging, The Ohio State University, 2Department of Psychology, University of Southern California, 3Biomedical Engineering, University of Southern California, 4Neuroscience Graduate Program, University of Southern California, 5Department of Psychology, The Ohio State University
Conventional computer hardware can not generate visual stimuli with sufficiently high grayscale resolution and measure response times with sufficient accuracy. We describe how to use the VideoSwitcher to produce high-resolution monochromatic displays, and the RTbox to measure response times with high accuracy on conventional computer hardware.
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Method for Novel Anti-Cancer Drug Development using Tumor Explants of Surgical Specimens
Kaushal Joshi1, Habibe Demir1, Ryosuke Yamada1, Takeshi Miyazaki1, Abhik Ray-Chaudhury2, Ichiro Nakano1
1Department of Neurological Surgery, The Ohio State University Medical Center, 2Department of Pathology, The Ohio State University Medical Center
Here, we established a method for drug efficacy testing with surgical specimens of brain tumors, termed “tumor explant method”. With this method, we can evaluate drug efficacy without breaking the microenvironment of solid tumors. To validate reliability of this method, we describe representative data with our glioma specimen treated with the current first-line chemotherapeutic agent, temozolomide.
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Use of an Optical Trap for Study of Host-Pathogen Interactions for Dynamic Live Cell Imaging
Jenny M. Tam1, Carlos E. Castro2, Robert J. W. Heath3, Michael K. Mansour1, Michael L. Cardenas1, Ramnik J. Xavier3, Matthew J. Lang4, Jatin M. Vyas1
1Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Harvard Medical School, 2Department of Mechanical and Aerospace Engineering, The Ohio State University, 3Center for Computational and Integrative Biology, Massachusetts General Hospital, Harvard Medical School, 4Dept. of Chemical and Biomolecular Engineering, Vanderbilt University
A method is described to individually select, manipulate, and image live pathogens using an optical trap coupled to a spinning disk microscope. The optical trap provides spatial and temporal control of organisms and places them adjacent to host cells. Fluorescence microscopy captures dynamic intercellular interactions with minimal perturbation to cells.
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A Method for Murine Islet Isolation and Subcapsular Kidney Transplantation
Erik J. Zmuda1, Catherine A. Powell1,2, Tsonwin Hai1,2,3
1Molecular and Cellular Biochemistry, Center for Molecular Neurobiology, The Ohio State University, 2Integrated Biomedical Science Graduate Program, The Ohio State University, 3Comprehensive Cancer Center, The Ohio State University
Transplantation of isolated islets has been proposed to be a potential treatment for type 1 diabetes. Here we describe a method to isolate islets from mouse pancreata and transplant them to the subcapsular space of the kidney.
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Dual-mode Imaging of Cutaneous Tissue Oxygenation and Vascular Function
Ronald X. Xu1, Kun Huang2, Ruogu Qin1, Jiwei Huang1, Jeff S. Xu1, Liya Ding2, Urmila S. Gnyawali3, Gayle M. Gordillo3, Surya C. Gnyawali3,4, Chandan K. Sen3
1Department of Biomedical Engineering, The Ohio State University, 2Department of Biomedical Informatics, The Ohio State University, 3Comprehensive Wound Center, The Ohio State University, 4Department of Surgery, The Ohio State University
A dual-mode imaging system was developed for non-contact assessment of cutaneous tissue oxygenation and vascular function.
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High-frequency High-resolution Echocardiography: First Evidence on Non-invasive Repeated Measure of Myocardial Strain, Contractility, and Mitral Regurgitation in the Ischemia-reperfused Murine Heart
Surya C. Gnyawali1,2, Sashwati Roy1,2, Jason Driggs1,2, Savita Khanna1,2, Thomas Ryan2,3, Chandan K. Sen2
1Department of Surgery, The Ohio State University, 2Heart and Lung Research Institute, The Ohio State University, 3Department of Cardiovascular Medicine, The Ohio State University
High frequency Doppler ultrasound is a novel technology for assessing regional myocardial function. This work presents first evidence demonstrating applicability of this versatile imaging platform for the repeated measure of myocardial strain, dp/dt, and mitral regurgitation in the ischemia-reperfused (IR) murine heart.
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