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Journal / Neuroscience / Elektroporatie van de hindbrain om Axonale…
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Neuroscience

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JoVE Journal Neuroscience
Elektroporatie van de hindbrain om Axonale Trajectories en Synaptic Doelen Trace in de Chick Embryo
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Elektroporatie van de hindbrain om Axonale Trajectories en Synaptic Doelen Trace in de Chick Embryo

Article DOI: 10.3791/50136-v • 10:04 min • May 29th, 2013
May 29th, 2013 •
1, 2, 2, 1
1Koret School of Veterinary Medicine, The Hebrew University of Jerusalem, 2Department of Medical Neurobiology, The Hebrew University of Jerusalem

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Hoe neuronale netwerken zijn in de embryonale hersenen opgericht is een fundamentele vraag in ontwikkelingsstoornissen neurobiologie. Hier combineerden we een electroporatietechniek met nieuwe genetische hulpmiddelen, zoals Cre / Lox-plasmiden en piggyBac-gemedieerde DNA omzetting systeem in de aviaire hindbrain om dorsale interneurons labelen en volgen hun axonale projecties en synaptische doelen op verschillende ontwikkelingsstadia.

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Neurowetenschappen Neurobiologie Ontwikkelingsbiologie Cellular Biology Moleculaire Biologie Anatomie Fysiologie genetica Electroporation Chick hindbrain Axon Interneuron DA1 piggyBac Enhancer Synapse neuronen axonen GFP expressie, Embryonale hindbrain hersenen diermodel
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