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Journal / Biology / Isolering og Kv-optagelser i murine atrielle og…
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JoVE Journal Biology
Isolering og Kv-optagelser i murine atrielle og ventrikulære cardiomyocytter
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Isolering og Kv-optagelser i murine atrielle og ventrikulære cardiomyocytter

Article DOI: 10.3791/50145-v • 11:33 min • March 12th, 2013
March 12th, 2013 •
1,2, 1, 1, 1, 1, 1,3
1Experimental and Clinical Research Center (ECRC), Charité Medical Faculty and Max-Delbrück Center for Molecular Medicine (MDC), 2Medical Department, Division of Cardiology, Campus Virchow-Klinikum, Charité - Universitätsmedizin Berlin, 3Medical Department, Division of Cardiology and Angiology, Campus Mitte, Charité - Universitätsmedizin Berlin

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Kv-kanal dysfunktion er forbundet med hjertearytmier. For at undersøge de molekylære mekanismer, der fører til sådanne arytmier vi benytter en systematisk protokol til isolering af atriale og ventrikulære kardiomyocytter fra Kv channel accessoriske subunit-knockout-mus. Isolerede cardiomyocytter kan derefter straks anvendes til cellulære elektrofysiologiske undersøgelser, biokemiske eller immunofluorescens (IF)-assays.

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Fysiologi Medicin Cellular Biology molekylærbiologi genetik Biomedical Engineering Anatomi Cardiology minutvolumen Low kardiomyopati hjertesvigt arytmi Cardiac ventrikulær dysfunktion cardiomyocytter Kv kanal hjertearytmi elektrofysiologi patch klemme mus dyremodel
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