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Journal / Biology / Isolering af murin atriale og ventrikulære…
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Biology

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JoVE Journal Biology
Isolering af murin atriale og ventrikulære myocytter af høj kvalitet til samtidige målinger afCa2+ transienter og L-type calciumstrøm
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Isolering af murin atriale og ventrikulære myocytter af høj kvalitet til samtidige målinger afCa2+ transienter og L-type calciumstrøm

Article DOI: 10.3791/61964-v • 06:22 min • November 3rd, 2020
November 3rd, 2020 •
*1,2,3, *1,2,3, 1,2, *1,2,3, *4,5,6
1Department of Medicine I, University Hospital Munich, Campus Großhadern, Ludwig-Maximilians University Munich (LMU), 2Partner Site Munich, Munich Heart Alliance (MHA), DZHK (German Centre for Cardiovascular Research), 3Walter Brendel Center of Experimental Medicine, Ludwig-Maximilians University Munich (LMU), 4Institute of Pharmacology and Toxicology, University Medical Center Göttingen, 5Partner Site Göttingen, DZHK (German Centre for Cardiovascular Research), 6Cluster of Excellence "Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells" (MBExC), University of Göttingen
* These authors contributed equally

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Musmodeller gør det muligt at studere nøglemekanismer for arytmogenese. Til dette formål er kardiomyocytter af høj kvalitet nødvendige for at udføre patch-clamp-målinger. Her beskrives en metode til isolering af murine atriale og ventrikulære myocytter via retrograd enzymbaseret Langendorff-perfusion, som muliggør samtidige målinger af calciumtransienter og L-type calciumstrøm.

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Denne måned i JoVE udgave 165 arytmi murin myocytisolering Langendorff-perfusion L-type calciumstrøm calciumtransient patchklemme
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