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Naïve Adult Stem Cells Isolation from Primary Human Fibroblast Cultures
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Naïve Adult Stem Cells Isolation from Primary Human Fibroblast Cultures

Naïve Adult Stem Cells Isolation from Primary Human Fibroblast Cultures

DOI:
10.3791/50185-v

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07:39 min

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May 03, 2013

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, , ,
1Department of Dermatology and Institute for Medical Engineering,Technische Universität München, 2Department of Dermatology and Allergology,Technische Universität München

Chapters

  • 00:05Title
  • 01:27SKP Isolation from Primary Fibroblast Cultures
  • 02:11Sphere Culture Conditions
  • 02:57Immunocytochemistry for Stem Cell Markers
  • 03:59Realtime PCR Analysis of Expression Levels of Stem Cell Markers
  • 05:01Directed Differentiation into Smooth Muscle Cells
  • 05:54Results: SKP Spheres from Primary Fibroblast Cultures
  • 07:16Conclusion

Summary

Automatic Translation

Automatic Translation

We report a method to isolate naïve multipotent skin-derived precursor (SKP) cells from primary human fibroblast cultures. We show that these SKPs derived from fibroblast cultures share similar stem cell properties to the ones derived directly from human skin biopsies. These cells express the neural crest marker, nestin, in addition to the multipotent markers such as OCT4 and Nanog.

Tags

Keywords: Adult Stem CellsSKPsSkin-derived PrecursorsPrimary Fibroblast CulturesMultipotent Precursor CellsDermal PrecursorsNestinVimentinFibronectinStem Cell MarkersGenetic DiseasesDisease Modeling

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