Mesure de transport de cations Na, K et H, K-ATPase dans<em> Xenopus</em> Les ovocytes par spectrophotométrie d'absorption atomique: une alternative à dosages radio-isotopes
We describe a method to quantify the activity of K+-countertransporting P-type ATPases by heterologous expression of the enzymes in Xenopus oocytes and measuring Rb+ or Li+ uptake into individual cells by atomic absorption spectrophotometry. The method is a sensitive and safe alternative to radioisotope flux experiments facilitating complex kinetic studies.