Meten kationtransport van Na, K en H, K-ATPase in<em> Xenopus</em> Eicellen door atomaire absorptiespectrometrie: Een alternatief voor Radio-isotopen Assays
We describe a method to quantify the activity of K+-countertransporting P-type ATPases by heterologous expression of the enzymes in Xenopus oocytes and measuring Rb+ or Li+ uptake into individual cells by atomic absorption spectrophotometry. The method is a sensitive and safe alternative to radioisotope flux experiments facilitating complex kinetic studies.