Mätning Cation Transport med Na, K-och H, K-ATPas i<em> Xenopus</em> Oocyter med atomabsorptionsspektrofotometri: Ett alternativ till radioisotoper Analyser
We describe a method to quantify the activity of K+-countertransporting P-type ATPases by heterologous expression of the enzymes in Xenopus oocytes and measuring Rb+ or Li+ uptake into individual cells by atomic absorption spectrophotometry. The method is a sensitive and safe alternative to radioisotope flux experiments facilitating complex kinetic studies.