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Journal / Medicine / Protocollo migliorato per microdissezione laser…
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JoVE Journal Medicine
Protocollo migliorato per microdissezione laser di isole pancreatiche umane da campioni chirurgici
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Protocollo migliorato per microdissezione laser di isole pancreatiche umane da campioni chirurgici

Article DOI: 10.3791/50231 • 09:44 min
January 6th, 2013 •
1,2, 3, 1,2, 1, 2, 1,2, 2, 4, 5, 6, 7, 8, 9, 2, 10, 3, 1
1Molecular Diabetology, Paul Langerhans Institute Dresden, 2Department of GI-, Thoracic- and Vascular Surgery, University Hospital Carl Gustav Carus, University of Technology Dresden, 3Department of Endocrinology and Metabolism, Metabolic Unit University of Pisa, 4Labs DC0522, Lilly Corporate Center, 5Genomics, Faculty of Medicine Imperial College London, 6Vital-IT, SIB Swiss Institute of Bioinformatics, 7Clinical Biochemistry, Hannover Medical School, 8Cell Physiology and Metabolism, Medical School, University of Geneva, 9Department of Pathology, University Hospital Carl Gustav Carus, University of Technology Dresden, 10R&D DIAB Division / Translational Medicine, Sanofi-Aventis

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January 6th, 2013

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Microdissezione laser è una tecnica che consente il recupero di cellule scelte da piccole quantità di parenchima. Qui si descrive un protocollo per l'acquisizione di isole pancreatiche umane da campioni chirurgici da utilizzare per gli studi di trascrittomica. Il nostro protocollo migliora l'autofluorescenza intrinseca delle cellule beta umane, facilitando così la loro collezione.

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