Improved Protocol For Laser Microdissection Of Human Pancreatic Islets From Surgical Specimens

Doroth&#233;e Sturm; Lorella Marselli; Florian Ehehalt; Daniela Richter; Marius Distler; Stephan Kersting; Robert Gr&#252;tzmann; Krister Bokvist; Philippe Froguel; Robin Liechti; Anne J&#246;rns; Paolo Meda; Gustavo Bruno Baretton; Hans-Detlev Saeger; Anke M. Schulte; Piero Marchetti; Michele Solimena

doi:10.3791/50231

Journal

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Medicine

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Protocolo melhorado para microdissecção a laser de ilhotas pancreáticas humanas a partir de espécimes cirúrgicos

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Medicine
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JoVE Journal Medicine

Improved Protocol For Laser Microdissection Of Human Pancreatic Islets From Surgical Specimens

Protocolo melhorado para microdissecção a laser de ilhotas pancreáticas humanas a partir de espécimes cirúrgicos

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14,293 Views

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09:43 min

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January 06, 2013

DOI:

10.3791/50231-v

DOI:

10.3791/50231-v

•

09:43 min

January 06, 2013

•

2 Views

Dorothée Sturm^1,2, Lorella Marselli³, Florian Ehehalt^1,2, Daniela Richter¹, Marius Distler², Stephan Kersting^1,2, Robert Grützmann², Krister Bokvist⁴, Philippe Froguel⁵, Robin Liechti⁶, Anne Jörns⁷, Paolo Meda⁸, Gustavo Bruno Baretton⁹, Hans-Detlev Saeger², Anke M. Schulte¹⁰, Piero Marchetti³, Michele Solimena¹

¹Molecular Diabetology,Paul Langerhans Institute Dresden, ²Department of GI-, Thoracic- and Vascular Surgery, University Hospital Carl Gustav Carus,University of Technology Dresden, ³Department of Endocrinology and Metabolism,Metabolic Unit University of Pisa, ⁴Labs DC0522,Lilly Corporate Center, ⁵Genomics,Faculty of Medicine Imperial College London, ⁶Vital-IT,SIB Swiss Institute of Bioinformatics, ⁷Clinical Biochemistry,Hannover Medical School, ⁸Cell Physiology and Metabolism, Medical School,University of Geneva, ⁹Department of Pathology, University Hospital Carl Gustav Carus,University of Technology Dresden, ¹⁰R&D DIAB Division / Translational Medicine,Sanofi-Aventis

Summary

Microdissecção a laser é uma técnica que permite a recuperação de células selecionadas a partir de pequenas quantidades de parênquima. Aqui descrevemos um protocolo de aquisição ilhotas pancreáticas humanas a partir de espécimes cirúrgicos para serem utilizados para estudos de transcriptomic. Nosso protocolo melhora a autofluorescência intrínseca das células beta humanas, facilitando assim a sua coleção.