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Immunology and Infection

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JoVE Journal Immunology and Infection
Monitoring Dendritic Cell Migration using 19F / 1H Magnetic Resonance Imaging
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Monitoring Dendritic Cell Migration using 19F / 1H Magnetic Resonance Imaging

Article DOI: 10.3791/50251-v • 08:12 min • March 20th, 2013
March 20th, 2013 •
1,2, 1,2, 1,2, 1,2, 2, 1,2, 1,2
1Experimental and Clinical Research Center, A joint cooperation between the Charité Medical Faculty and the Max Delbrück Center for Molecular Medicine, 2Berlin Ultrahigh Field Facility (B.U.F.F.), Max Delbrück Center for Molecular Medicine

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Tracking of cells using MRI has gained remarkable attention in the past years. This protocol describes the labeling of dendritic cells with fluorine (19F)-rich particles, the in vivo application of these cells, and monitoring the extent of their migration to the draining lymph node with 19F/1H MRI and 19F MRS.

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Dendritic Cell Migration 19F MRI Noninvasive Imaging Contrast Agents MR Relaxation Times T1 Enhancement T2 Depletion Cell Labeling Strategies Ultrasmall Iron Oxide Agents FDA Approval T2 Agents Drawback Fluorine-rich Particles Perfluorocarbon Compounds 19F MR Spectroscopy
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