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JoVE Journal
Biology

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从解离分析准备胚胎和幼体斑马鱼骨肌纤维
 
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从解离分析准备胚胎和幼体斑马鱼骨肌纤维

Article DOI: 10.3791/50259-v 05:58 min November 13th, 2013
November 13th, 2013

Chapters

Summary

Please note that all translations are automatically generated.

Click here for the English version.

斑马鱼是用于模拟骨骼肌的人类疾病的一个新兴的系统。我们描述了一个快速,高效的方法从胚胎和幼虫的斑马鱼隔离骨骼肌肌纤维。这种方法产生了高密度的肌纤维制备适合的单骨骼肌纤维形态的研究中,蛋白质的亚细胞定位,以及肌肉生理学。

Tags

基本协议,发行81,斑马鱼,神经肌肉疾病,肌肉疾病,肌营养不良,原代细胞培养,免疫组化(IHC),骨骼肌,肌纤维,实时成像
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