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在体内病态的人类基因组建模使用斑马鱼
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
In Vivo Modeling of the Morbid Human Genome using Danio rerio
DOI:

12:31 min

August 24, 2013

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Chapters

  • 00:05Title
  • 01:20Bioinformatics and Expressions Analysis in the Developing Zebrafish Embryo
  • 02:27Site-directed Mutagenesis and in vitro mRNA Transcription
  • 03:54In vivo Assay of Variant Loss of Function
  • 06:40In vivo Assay of Variant Dominant Negative or Gain of Function Effects
  • 08:11Representative MO Knockdown and Rescue Results
  • 12:06Conclusion

Summary

Automatic Translation

在这里,我们提出了一个系统的方法来开发有关生理,敏感,特异<em>在体内</em>分析用于解释人类病理学变化。瞬态的遗传操作,通过微量注射野生型和突变型人mRNA和吗啉代(MO)的反义寡核苷酸利用显影斑马鱼胚胎的快速检测致病突变,特别是,但不完全是,在人类发育障碍的上下文中的易处理性。

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