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Biology

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对小鼠胰腺胰岛分离和细胞内cAMP的测定方法
 
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对小鼠胰腺胰岛分离和细胞内cAMP的测定方法

Article DOI: 10.3791/50374
June 25th, 2014

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Summary June 25th, 2014

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含量测定采用胰岛分离的小鼠胰岛体外 β细胞功能是在糖尿病病理生理学和治疗学研究的一个重要组成部分。虽然许多下游应用的情况下,该协议特别描述作为必要参数确定的β-细胞功能的细胞内环磷酸腺苷(cAMP)的测定。

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