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小鼠胰岛的朗格汉斯的分离纯化的胶原酶和中性蛋白酶结合使用
JoVE Journal
Biology
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JoVE Journal
Biology
Mouse Islet of Langerhans Isolation using a Combination of Purified Collagenase and Neutral Protease
Please note that all translations are automatically generated.
Click here for the English version.
小鼠胰岛的朗格汉斯的分离纯化的胶原酶和中性蛋白酶结合使用
DOI:
10.3791/4137-v
•
06:59 min
•
September 07, 2012
•
Natalie D. Stull
,
Andrew Breite
,
Robert McCarthy
,
Sarah A. Tersey
,
Raghavendra G. Mirmira
3,4
1
Department of Pediatrics and the Herman B Wells Center for Pediatric Research
,
Indiana University School of Medicine
,
2
VITACYTE, LLC
,
3
Department of Medicine
,
Indiana University School of Medicine
,
4
Department of Cellular and Integrative Physiology
,
Indiana University School of Medicine
Chapters
00:05
Title
01:20
Inflation of Pancreas with Collagenase/Protease Mixture
03:58
Pancreas Dissociation
05:49
Results: Islet Isolation
06:35
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
小鼠胰岛分离的详细描述描述原位胰腺导管插管和灌注纯化的胶原酶和中性蛋白酶的组合中使用的技术。
Tags
Mouse Islet Of Langerhans Isolation
Purified Collagenase
Neutral Protease
Beta Cell Gene Expression
Intracellular Signaling Pathways
Secretory Responses
Islet Yield
Islet Quality
Rodent Strain
Lot-to-lot Variations
Dose Of Enzyme
Purified TDEs
Rodent Cell Isolations
Human Islet Isolations
VitaCyte LLC
Modified Mouse Islet Isolation Protocol
Pancreatic Duct
Histopaque Gradient
Article
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