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Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics
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Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics

Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics

DOI:
10.3791/64792-v

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09:09 min

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December 09, 2022

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, , ,
1Helmholtz Pioneer Campus (HPC),Helmholtz Munich, 2Institute of Computational Biology, Computational Health Department,Helmholtz Munich, 3Division of Infectious Diseases and Tropical Medicine,Ludwig-Maximillian-Universität Klinikum, 4TUM School of Medicine,Technical University of Munich

Chapters

  • 00:04Introduction
  • 00:57Nuclei Isolation
  • 05:11Nuclei Sorting for Hepatocyte Ploidy Profiling or Well-Based Sequencing Approaches
  • 06:36Results: Isolation of Nuclei from Flash-Frozen, Archived Liver Tissues for Single-Nucleus RNA-Seq, ATAC-Seq, and Joint Multiomics
  • 08:39Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol for isolating nuclei from flash-frozen, archived liver tissues for single-nucleus RNA-seq, ATAC-seq, and joint multiomics (RNA-seq and ATAC-seq).

Tags

Keywords: Nuclei IsolationSingle-cell MultiomicsDensity Gradient CentrifugationIodixanolCell StrainerBio-archived Human Liver SamplesMouse ModelNon-human Primate

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