11,886 Views
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13:22 min
September 10, 2013
DOI:
10.3791/50439-v
グルココルチコイド応答インビボゼブラルシフェラーゼ活性(GRIZLY)アッセイ:私たちは、ゼブラフィッシュの幼虫を使用したグルココルチコイドストレスホルモンのシグナル伝達のための化学スクリーニングシステムの手順とデータ解析について説明します。アッセイは、高感度かつ特異的に代謝を必要とするか、内因性のグルココルチコイドの産生に影響する化合物によってグルココルチコイドシグナル伝達への影響を検出します。
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Live Imaging of Innate Immune and Preneoplastic Cell Interactions Using an Inducible Gal4/UAS Expression System in Larval Zebrafish Skin
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Cite this Article
Weger, B. D., Weger, M., Jung, N., Lederer, C., Bräse, S., Dickmeis, T. A Chemical Screening Procedure for Glucocorticoid Signaling with a Zebrafish Larva Luciferase Reporter System. J. Vis. Exp. (79), e50439, doi:10.3791/50439 (2013).
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