A Chemical Screening Procedure for Glucocorticoid Signaling with a Zebrafish Larva Luciferase Reporter System

Benjamin D. Weger; Meltem Weger; Nicole Jung; Christin Lederer; Stefan Br&#228;se; Thomas Dickmeis

doi:10.3791/50439

Journal

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Biology

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Bir Zebra balığı Larva Lusıferaz Muhabir Sistemi ile glukokortikoid Signaling'den için bir Kimyasal Tarama Prosedürü

A Chemical Screening Procedure for Glucocorticoid Signaling with a Zebrafish Larva Luciferase Reporter System

JoVE Journal
Biology

This content is Free Access.

JoVE Journal Biology

A Chemical Screening Procedure for Glucocorticoid Signaling with a Zebrafish Larva Luciferase Reporter System

Bir Zebra balığı Larva Lusıferaz Muhabir Sistemi ile glukokortikoid Signaling'den için bir Kimyasal Tarama Prosedürü

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11,923 Views

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13:22 min

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September 10, 2013

DOI:

10.3791/50439-v

DOI:

10.3791/50439-v

•

13:22 min

September 10, 2013

•

11911 Views

Benjamin D. Weger¹, Meltem Weger¹, Nicole Jung², Christin Lederer¹, Stefan Bräse^1,3, Thomas Dickmeis¹

¹Institute of Toxicology and Genetics,Karlsruhe Institute of Technology – Campus North ²Institute of Organic Chemistry,Karlsruhe Institute of Technology – Campus North ³Institute of Organic Chemistry,Karlsruhe Institute of Technology – Campus South

Summary

We describe the procedure and data analysis of a chemical screening system for glucocorticoid stress hormone signaling using zebrafish larvae: the Glucocorticoid Responsive In vivo Zebrafish Luciferase activitY (GRIZLY) assay. The assay sensitively and specifically detects effects on glucocorticoid signaling by compounds that require metabolization or affect endogenous glucocorticoid production.