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JoVE Journal
Biology

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对糖皮质激素信号A化学筛选程序与斑马鱼幼虫的荧光素酶报告系统
 
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对糖皮质激素信号A化学筛选程序与斑马鱼幼虫的荧光素酶报告系统

Article DOI: 10.3791/50439-v 13:22 min September 10th, 2013
September 10th, 2013

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Summary

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Click here for the English version.

我们描述了利用斑马鱼幼虫糖皮质激素的应激激素信号的化学筛选系统的程序和数据分析:糖皮质激素响应斑马鱼体内的荧光素酶活性(GRIZLY)检测。该法灵敏,特别是通过需要代谢或影响内源性糖皮质激素的生产检测化合物对糖皮质激素信号传导的影响。

Tags

发育生物学,79期,生物化学,脊椎动物,斑马鱼,环境影响(生物和动物),遗传学(动物),生命科学,动物生物学,动物模型,生物化学,生物工程(总称),激素,激素替代,和激素拮抗剂,斑马鱼,
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