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JoVE Journal
Biology

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对糖皮质激素信号A化学筛选程序与斑马鱼幼虫的荧光素酶报告系统
 
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对糖皮质激素信号A化学筛选程序与斑马鱼幼虫的荧光素酶报告系统

Article DOI: 10.3791/50439
September 10th, 2013

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Summary September 10th, 2013

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我们描述了利用斑马鱼幼虫糖皮质激素的应激激素信号的化学筛选系统的程序和数据分析:糖皮质激素响应斑马鱼体内的荧光素酶活性(GRIZLY)检测。该法灵敏,特别是通过需要代谢或影响内源性糖皮质激素的生产检测化合物对糖皮质激素信号传导的影响。

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