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Journal
/
Bioengineering
/
一个台式生物反应器的操作
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
Operation of a Benchtop Bioreactor
Please note that all translations are automatically generated.
Click here for the English version.
一个台式生物反应器的操作
DOI:
10.3791/50582-v
•
12:54 min
•
September 12, 2013
•
Kristina M. Obom
,
Andrew Magno
,
Patrick J. Cummings
1
Center for Biotechnology Education, Krieger School of Arts and Sciences
,
Johns Hopkins University
,
2
ATR Biotech
Chapters
00:05
Title
01:40
Fermentor Vessel Set-up
02:31
Fermentor Vessel Set-up: pH Probe Calibration
03:49
Fermentor Vessel Set-up: Continuation of Vessel Set-up and Calibration of pO
2
Probe
08:01
Fermentor Vessel Set-up: Inoculation and Sampling
10:03
Results: A Typical Bioreactor Run
12:07
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
发酵罐是用来增加培养产量生物工程化细胞和生产率。在摇瓶筛选多种微生物或动物细胞培养人选后,下一个合乎逻辑的步骤是增加了选择的文化的生物质发酵罐。该视频演示了一个典型的台式生物反应器系统的设置和操作。
Tags
Fermentation System
Cell Culture
Bioreactor
Temperature Control
PH Control
Dissolved Oxygen
Fermentor
Shake Flask
Benchtop Fermentation
Sterilization
Inoculation
Growth Medium
Sampling
Harvesting
Data Analysis
System Cleanup
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