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Journal / Biology / Derribo Gene a gran escala en
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Derribo Gene a gran escala en
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Derribo Gene a gran escala en

Article DOI: 10.3791/50693 • 18:38 min
September 25th, 2013 •
1, 2, 3
1Molecular and Cellular Biology Program, University of Massachusetts, Amherst, 2Neuroscience and Behavior Program, University of Massachusetts, Amherst, 3Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst

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September 25th, 2013

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Mientras dsRNA alimentación en C. elegans es una poderosa herramienta para evaluar la función de los genes, los protocolos actuales para pantallas de alimentación a gran escala resultan en eficiencias desmontables variables. Se describe un protocolo mejorado para realizar a gran escala pantallas de alimentación de RNAi que se traduce en caída altamente eficaz y reproducible de la expresión génica.

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