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使用普洛林来评估阿克森指导受体在细胞培养和小鸡胚胎中的动力学
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JoVE Journal
Neuroscience
Use of pHluorin to Assess the Dynamics of Axon Guidance Receptors in Cell Culture and in the Chick Embryo
Please note that all translations are automatically generated.
Click here for the English version.
使用普洛林来评估阿克森指导受体在细胞培养和小鸡胚胎中的动力学
DOI:
10.3791/50883-v
•
10:30 min
•
January 12, 2014
•
Céline Delloye-Bourgeois*
1
,
Arnaud Jacquier*
1
,
Julien Falk
,
Valérie Castellani
1
University Claude Bernard Lyon1, CGphiMC UMR CNRS 5534
,
University of Lyon
Chapters
00:05
Title
02:05
Transfecting COS7 Cells
05:26
In ovo
Electroporation of pHluorin-PlexinA1 Construct
08:20
Expression of pHluorin-PlexinA1
09:26
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们在这里描述了使用pH敏感的绿色荧光蛋白变种,pHluorin,来研究在细胞表面贩运的轴向制导受体的间歇性时间动力学。pHluorin标记的受体在细胞培养和
体内
都表达,使用小鸡胚胎的电波。
Tags
PHluorin
Axon Guidance Receptors
Cell Culture
Chick Embryo
Spatio-temporal Dynamics
Cell Surface Dynamics
Growth Cones
Signaling Mechanisms
PH-dependent Fluorescence
GFP Variant
Plasma Membrane
In Vitro
In Vivo
Spinal Cord
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