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Isolation and Culture of Chick Ciliary Ganglion Neurons
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Neuroscience
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JoVE Journal Neuroscience
Isolation and Culture of Chick Ciliary Ganglion Neurons

Isolation and Culture of Chick Ciliary Ganglion Neurons

DOI:
10.3791/61431-v

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14:36 min

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August 08, 2020

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, , , ,
1Institute of Biomedicine, Department of Medical Sciences – iBiMED,University of Aveiro, 2CNC – Center for Neuroscience and Cell Biology,University of Coimbra

Chapters

  • 00:00Introduction
  • 01:41Preparation of Coverslips
  • 08:00Dissociation and Culture of Chick Ciliary Ganglion Neurons
  • 11:42Representative Results
  • 12:42Discussion and Conclusion

Summary

Automatic Translation

Automatic Translation

Chick ciliary ganglia (CG) are part of the parasympathetic nervous system. Neuronal cultures of chick CG neurons were shown to be effective cell models in the study of nerve muscle interactions. We describe a detailed protocol for the dissection, dissociation and in vitro culture of CG neurons from chick embryos.

Tags

Keywords: Chick Ciliary GanglionCiliary Ganglion NeuronsParasympathetic Nervous SystemCholinergic NeuronsCholinergic SynapsesCiliary NeuronsChoroidal NeuronsNeuromuscular SynapsesCiliary Ganglion DissectionPrimary Cell CultureGlass Coverslip Preparation

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