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肺动脉平滑肌细胞从新生小鼠隔离
JoVE Journal
Biology
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JoVE Journal
Biology
Isolation of Pulmonary Artery Smooth Muscle Cells from Neonatal Mice
Please note that all translations are automatically generated.
Click here for the English version.
肺动脉平滑肌细胞从新生小鼠隔离
DOI:
10.3791/50889-v
•
08:02 min
•
October 19, 2013
•
Keng Jin Lee
,
Lyubov Czech
,
Gregory B. Waypa
,
Kathryn N. Farrow
1
Department of Pediatrics
,
Northwestern University Feinberg School of Medicine
Chapters
00:05
Title
01:25
Pulmonary Artery (PA) Isolation from Neonatal Mice
03:27
Culturing Pulmonary Artery Smooth Muscle Cells (PASMC)
06:30
Results: Pulmonary Artery Smooth Muscle Cells (PASMC) Expression
07:32
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们已经开发了一种新的和可重复的技术来隔离为P7作为年轻的小鼠原代培养的肺动脉平滑肌细胞(PASMC),从而使更好地参与新生儿平滑肌细胞的收缩和舒张的信号转导通路的研究。
Tags
Pulmonary Artery Smooth Muscle Cells
Pulmonary Hypertension
Neonatal Mice
Cell Isolation
Agarose
Iron Particles
Collagenase
Cell Culture
Smooth Muscle Cell Markers
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