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Immunology and Infection

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JoVE Journal Immunology and Infection
Kvantitativ vurdering af human neutrofil migration over en Kulturperler Blære epitel
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Kvantitativ vurdering af human neutrofil migration over en Kulturperler Blære epitel

Article DOI: 10.3791/50919-v • 11:20 min • November 7th, 2013
November 7th, 2013 •
1, 1,2
1Department of Pediatrics, Washington University School of Medicine, 2Department of Molecular Microbiology, Washington University School of Medicine

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Vi har udviklet en in vitro-model, der efterligner en vigtig komponent i den akutte inflammatoriske respons under infektion af blæren med uropatogen Escherichia coli. Den transuroepithelial neutrofil migration assay muliggør kvantitativ vurdering af human neutrofil migration over blæren epitel, dyrket på permeable understøtninger, som reaktion på bakteriel infektion eller kemotiltrækkende stoffer.

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Immunologi uropatogen blære epitel neutrofilmigration medfødte immunitet urinvejsinfektion
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