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In vitro Coculture Assay at vurdere Patogen induceret Neutrophil Trans-epitel Migration
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Immunology and Infection
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JoVE Journal Immunology and Infection
In vitro Coculture Assay to Assess Pathogen Induced Neutrophil Trans-epithelial Migration

In vitro Coculture Assay at vurdere Patogen induceret Neutrophil Trans-epitel Migration

DOI:
10.3791/50823-v

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14:14 min

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January 06, 2014

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, , ,
1Department of Pediatrics,Harvard Medical School, 2Department of Pediatric Gastroenterology,MGH for Children, 3Mucosal Immunology and Biology Research Center,Massachusetts General Hospital

Chapters

  • 00:05Title
  • 02:01Collagen Coating Transwells
  • 03:25Seeding Collagen Coated Transwells with Epithelial Cells
  • 04:30Preparation of Epithelial Cell Layers for Migration Assay
  • 06:01Neutrophil Trans-epithelial Migration Assay
  • 11:27Results: Assessing Neutrophil Trans-epithelial Migration
  • 13:23Conclusion

Summary

Automatic Translation

Automatic Translation

Neutrofil trans-epitelmigration som reaktion på slimhindebakteriel infektion bidrager til epitelskader og kliniske sygdomme. En in vitro-model er blevet udviklet, der kombinerer patogen, humane neutrofiler og polariserede menneskelige epitelcellelag dyrket på transwellfiltre for at lette undersøgelser mod optrævling af de molekylære mekanismer, der orkestrerer dette fænomen.

Tags

In Vitro CocultureNeutrophil Trans-epithelial MigrationMucosal ImmunityPathogenic InfectionPolarized Epithelial MonolayerNeutrophil InfiltrationInflammatory ResponseTherapeutic Targets

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