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Journal / Neuroscience / Imaging spine dendritiche di Rat Primaria neuroni …
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JoVE Journal Neuroscience
Imaging spine dendritiche di Rat Primaria neuroni dell'ippocampo utilizzando Structured Illumination Microscopy
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Imaging spine dendritiche di Rat Primaria neuroni dell'ippocampo utilizzando Structured Illumination Microscopy

Article DOI: 10.3791/51276-v • 14:11 min • May 4th, 2014
May 4th, 2014 •
*1, *2, 1, 2, 1, 1, 1, 2, 1
1Center for Neuroscience, Swammerdam Institute for Life Sciences, University of Amsterdam, 2Van Leeuwenhoek Centre for Advanced Microscopy, Section Molecular Cytology, Swammerdam Institute for Life Sciences, University of Amsterdam
* These authors contributed equally

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Questo articolo descrive un protocollo di lavoro di immagine spine dendritiche di neuroni dell'ippocampo in vitro utilizzando Structured Illumination Microscopy (SIM). Microscopia super-risoluzione usando SIM fornisce risoluzione di immagine notevolmente oltre il limite di diffrazione della luce in tutte le tre dimensioni spaziali, permettendo l'imaging di singole spine dendritiche con maggiore dettaglio.

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Neuroscienze Numero 87 spine dendritiche Microscopia confocale fluorescenza Neuroscienze ippocampo neurone primario microscopia risoluzione super strutturato microscopia illuminazione (SIM) le neuroscienze dendriti
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