Journal
/
/
成像大鼠原代海马神经元树突棘使用结构照明显微镜
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy
DOI:

14:11 min

May 04, 2014

, , , , , , , ,

Chapters

  • 00:05Title
  • 02:05Coverslip Preparation
  • 02:48Dissection of the Hippocampi
  • 03:58Cell Dissociation and Plating
  • 05:35Rat Hippocampal Primary Neuron Transfection using Lipofectamine
  • 07:23Immunostaining Hippocampal Primary Neurons
  • 08:54Dendritic Spine Imaging using Structure Illumination Microscopy
  • 12:12Results: Dendrites and Dendritic Spines Imaged with SIM and Confocal Microscopy
  • 13:22Conclusion

Summary

Automatic Translation

本文介绍了使用结构照明显微镜(SIM卡) 在体外海马神经元的工作协议,以形象的树突棘。使用SIM卡超高分辨率显微镜提供的图像分辨率显著超越在所有三个空间维度的光的衍射极限,允许单个树突棘与改进的细节的影像。

Related Videos

Read Article