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Journal
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Neuroscience
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小脑轴突的激光Nanosurgery<em>在体内</em
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Laser Nanosurgery of Cerebellar Axons
In Vivo
Please note that all translations are automatically generated.
Click here for the English version.
小脑轴突的激光Nanosurgery<em>在体内</em
DOI:
10.3791/51371-v
•
09:25 min
•
July 28, 2014
•
Anna L. Allegra Mascaro
,
Leonardo Sacconi
2
,
Francesco Saverio Pavone
2,3,4
1
European Laboratory for Non-Linear Spectroscopy
,
University of Florence
,
2
National Institute of Optics
,
National Research Council
,
3
Department of Physics and Astronomy
,
University of Florence
,
4
International Center for Computational Neurophotonics (ICON Foundation)
Chapters
00:05
Title
02:02
Axonal Labeling
04:42
Optical Window on the Cerebellar Cortex
06:29
In Vivo
Multi-photon Laser Axotomy
07:56
Results: Laser Nanosurgery of Cerebllar Axons
In Vivo
08:44
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
双光子成像,其耦合到激光nanodissection,是有用的工具来研究退化和再生过程中对中枢神经系统具有亚细胞分辨率。该协议显示了如何标记,图像,并剖析单个登山纤维在
体内
小脑皮质。
Tags
Laser Nanosurgery
Cerebellar Axons
In Vivo
Two-photon Fluorescence Imaging
Neuronal Regeneration
Climbing Fibers
Olivocerebellar Neurons
Anterograde Tracing
Dextran-conjugated Dye
Cranial Window
Ti:Sapphire Laser
Article
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