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April 23, 2014
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Summary
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Complejos de proteínas con múltiples componentes juegan un papel crucial durante la función y el desarrollo celular. Aquí se describe un método usado para aislar los complejos de proteínas nativas a partir de embriones de Drosophila después de la reticulación en vivo seguido de la purificación de los complejos reticulados para el análisis de estructura-función subsiguiente.
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Cite this Article
Gao, M., McCluskey, P., Loganathan, S. N., Arkov, A. L. An in vivo Crosslinking Approach to Isolate Protein Complexes From Drosophila Embryos. J. Vis. Exp. (86), e51387, doi:10.3791/51387 (2014).
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