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囊性纤维化跨膜电导调节蛋白表达纯化<em>酿酒酵母</em
JoVE Journal
Biology
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Biology
Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein Expressed in
Saccharomyces cerevisiae
Please note that all translations are automatically generated.
Click here for the English version.
囊性纤维化跨膜电导调节蛋白表达纯化<em>酿酒酵母</em
DOI:
10.3791/51447-v
•
15:12 min
•
May 10, 2014
•
Naomi Pollock
,
Natasha Cant
,
Tracy Rimington
,
Robert C. Ford
1
Faculty of Life Sciences
,
University of Manchester
Chapters
00:05
Title
01:46
Solubilization of Microsomes
03:49
Nickel-affinity Purification of CFTR
05:33
Gel Permeation Chromatography (GPC) Purification of CFTR
06:57
Reconstitution of CFTR
07:51
Measurement of ATPase Activity
09:35
Results: Isolation of Milligram Quantities of CFTR by Two Methods
14:28
Conclusion
Summary
Automatic Translation
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Automatic Translation
异源表达和囊性纤维化跨膜电导调节器(CFTR)的净化是显著的挑战和制约因素的药物治疗囊性纤维化的发展。这个协议描述了两种方法的CFTR的毫克量适合于功能和结构研究的分离。
Tags
Cystic Fibrosis
CFTR Protein
Membrane Protein Purification
Saccharomyces Cerevisiae
Detergent Solubilization
DDM
LPG-14
ATPase Activity
Post-translational Modifications
Structural Analysis
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