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qPCR Is a Sensitive and Rapid Method for Detection of Cytomegaloviral DNA in Formalin-fixed, Paraffin-embedded Biopsy Tissue
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qPCR Is a Sensitive and Rapid Method for Detection of Cytomegaloviral DNA in Formalin-fixed, Paraffin-embedded Biopsy Tissue

Article DOI: 10.3791/51570-v • 08:08 min • July 9th, 2014
July 9th, 2014 •
1, 2, 2, 2, 1, 1, 1, 1, 1, 1
1Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, 2Department of Pathology and Laboratory Medicine, Indiana University Health

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Summary
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This protocol describes qPCR detection of cytomegalovirus in formalin-fixed, paraffin-embedded biopsy tissue, which is rapid, sensitive, specific, and useful for interpreting equivocal hematoxylin and eosin or immunohistochemical staining patterns.

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QPCR Cytomegaloviral DNA Formalin-fixed Paraffin-embedded Biopsy Tissue CMV Infection Gastrointestinal Tract Immunosuppressed Patients Laboratory Methods Serology Viral Culture Molecular Methods Histology Biopsy Tissue Hematoxylin And Eosin Staining Immunohistochemistry Atypical Staining Patterns Interpretation Difficult Quantitative Polymerase Chain Reaction High Sensitivity And Specificity FFPE Biopsy Tissue Clinical Laboratory Setting
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