This protocol describes qPCR detection of cytomegalovirus in formalin-fixed, paraffin-embedded biopsy tissue, which is rapid, sensitive, specific, and useful for interpreting equivocal hematoxylin and eosin or immunohistochemical staining patterns.
McCoy, M. H., Post, K., Sen, J. D., Chang, H. Y., Zhao, Z., Fan, R., Chen, S., Leland, D., Cheng, L., Lin, J. qPCR Is a Sensitive and Rapid Method for Detection of Cytomegaloviral DNA in Formalin-fixed, Paraffin-embedded Biopsy Tissue. J. Vis. Exp. (89), e51570, doi:10.3791/51570 (2014).