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Surgical Retrieval, Isolation and In vitro Expansion of Human Anterior Cruciate Ligament-derived Cells for Tissue Engineering Applications
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1Department of Medical Microbiology, Immunology & Cell Biology,Southern Illinois University School of Medicine, 2Division of Orthopaedics and Rehabilitation, Department of Surgery,Southern Illinois University School of Medicine, 3Department of Electrical and Computer Engineering, Biomedical Engineering Program,Southern Illinois University Carbondale, 4University of Illinois at Springfield
Chapters
- 00:05Title
- 01:23Tissue Digestion and hACL Isolation
- 02:16Cellular Expansion, Freezing and Thawing
- 03:26Fabrication of Tissue Engineered 2-D Poly Lactic-co-glycolic Acid (PLAGA) Scaffold
- 04:22Scanning Electron Microscopy (SEM)
- 05:54Immunofluorescence Staining
- 07:01Results: Cellular Adhesion and Morphology Confirm Biocompatibility on Scaffold Surface
- 08:02Conclusion
For future applications as a patch to repair partial tears of the Anterior Cruciate Ligament (ACL), human ACL derived cells were isolated from tissue obtained during reconstructive procedures, expanded in vitro and grown on tissue engineered scaffolds. Cellular adhesion and morphology was then performed to confirm biocompatibility on scaffold surface.
