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Recupero chirurgico, Isolamento e<em> In vitro</em> Ampliamento anteriore umana crociati Cells Legamento di derivazione per applicazioni di ingegneria tissutale
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1Department of Medical Microbiology, Immunology & Cell Biology,Southern Illinois University School of Medicine, 2Division of Orthopaedics and Rehabilitation, Department of Surgery,Southern Illinois University School of Medicine, 3Department of Electrical and Computer Engineering, Biomedical Engineering Program,Southern Illinois University Carbondale, 4University of Illinois at Springfield
Chapters
- 00:05Title
- 01:23Tissue Digestion and hACL Isolation
- 02:16Cellular Expansion, Freezing and Thawing
- 03:26Fabrication of Tissue Engineered 2-D Poly Lactic-co-glycolic Acid (PLAGA) Scaffold
- 04:22Scanning Electron Microscopy (SEM)
- 05:54Immunofluorescence Staining
- 07:01Results: Cellular Adhesion and Morphology Confirm Biocompatibility on Scaffold Surface
- 08:02Conclusion
Per le future applicazioni come una patch per riparare rotture parziali del legamento crociato anteriore (LCA), le cellule derivate ACL umane sono state isolate da tessuti ottenuti durante le procedure ricostruttive, espanse in vitro e coltivati in ingegneria tessutale ponteggi. Adesione cellulare e la morfologia è stata poi eseguita per confermare biocompatibilità sulla superficie patibolo.
