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Kirurgisk henting, isolasjon og<em> In vitro</em> Utvidelse av Menneskelig Fremre korsbånd-deriverte celler for tissue engineering applikasjoner
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1Department of Medical Microbiology, Immunology & Cell Biology,Southern Illinois University School of Medicine, 2Division of Orthopaedics and Rehabilitation, Department of Surgery,Southern Illinois University School of Medicine, 3Department of Electrical and Computer Engineering, Biomedical Engineering Program,Southern Illinois University Carbondale, 4University of Illinois at Springfield
Chapters
- 00:05Title
- 01:23Tissue Digestion and hACL Isolation
- 02:16Cellular Expansion, Freezing and Thawing
- 03:26Fabrication of Tissue Engineered 2-D Poly Lactic-co-glycolic Acid (PLAGA) Scaffold
- 04:22Scanning Electron Microscopy (SEM)
- 05:54Immunofluorescence Staining
- 07:01Results: Cellular Adhesion and Morphology Confirm Biocompatibility on Scaffold Surface
- 08:02Conclusion
For fremtidige søknader som en patch for å reparere delvis tårer av fremre korsbånd (ACL), ble menneske ACL avledet celler isolert fra vev innhentet under rekonstruktiv prosedyrer, utvidet in vitro og vokst på vev konstruert stillaser. Cellulær adhesjon og morfologi ble deretter utført for å bekrefte biokompatibilitet på stillaset overflaten.
