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结合<em>在体内</em>光学及μCT成像监视感染,炎症和骨解剖在骨科植入物感染的小鼠
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal
Medicine
Combined
In vivo
Optical and µCT Imaging to Monitor Infection, Inflammation, and Bone Anatomy in an Orthopaedic Implant Infection in Mice
Please note that all translations are automatically generated.
Click here for the English version.
结合<em>在体内</em>光学及μCT成像监视感染,炎症和骨解剖在骨科植入物感染的小鼠
DOI:
10.3791/51612-v
•
18:40 min
•
October 16, 2014
•
Nicholas M. Bernthal
,
Brad N. Taylor
,
Jeffrey A. Meganck
,
Yu Wang
,
Jonathan H. Shahbazian
,
Jared A. Niska
,
Kevin P. Francis
,
Lloyd S. Miller
4
1
Orthopaedic Hospital Research Center, Orthopaedic Hospital Department of Orthopaedic Surgery
,
David Geffen School of Medicine at University of California, Los Angeles (UCLA)
,
2
PerkinElmer
,
3
Department of Dermatology
,
Johns Hopkins University School of Medicine
,
4
Department of Medicine, Division of Infectious Diseases, Department of Orthopaedic Surgery
,
Johns Hopkins University School of Medicine
Chapters
00:05
Title
01:51
Mouse Surgical Procedure
03:56
2D Optical Imaging (In vivo Bioluminescent and Fluorescent Imaging)
06:35
µCT Image Acquisition
08:11
3D Optical Image Acquisition, Formation and µCT Co-registration
12:18
µCT Image Analysis
16:09
Results: Representative Bioluminescent and µCT Images
17:49
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
结合光学和μCT成像中的整形外科植入物感染的小鼠模型中,利用
金黄色葡萄球菌
的生物发光的工程应变
,
所提供的能力,以非侵入性和纵向监测细菌感染的动力学,以及相应的炎症反应和解剖学上的变化,在骨。
Tags
Multimodal Imaging
In Vivo Optical Imaging
MicroCT Imaging
Orthopaedic Implant Infection
Staphylococcus Aureus
Neutrophil
Inflammation
Osteomyelitis
Bone Anatomy
Mouse Model
Article
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